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Analysis of Heteroplasmy Among Mitochondrial Genomes at the Single Molecule Level using LATE-PCR and Allied Technologies
Conference presentation

Analysis of Heteroplasmy Among Mitochondrial Genomes at the Single Molecule Level using LATE-PCR and Allied Technologies

Lawrence J. Wangh and Adam Osborne
SELECTBIO Genomics Research 2013 (Boston, MA, 05/09/2013 - 05/10/2013)
05/09/2013

Abstract

Mitochondria Mitochondrial DNA PCR DNA Genomics
A LATE-PCR Lights-On/Lights-Off probe assay was developed to look for mutations in the mitochondrial genome. Exposure of both HepG2 and CCD-1112Sk cells to 3'-Azido-3'-deoxythymidine (AZT) caused a significant increase in mutations in regions of the HV2 and ND1 genes.

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