Abstract
Nanoarchitectonics, as a technology to arrange nano-sized structural units such as molecules in a desired configuration, requires nano-organization, which usually relies on intermolecular interactions, a phenomenon ubiquitous in biology but relatively less explored by chemists and materials scientists. During the past two decades, nanoarchitectures generated intracellularly, extracellularly, or intercellularly exhibit diverse range of functions and result in rapid development. This thesis focuses on using enzymatic reactions to control intermolecular interactions for generating artificial nanoarchitectures in a cellular environment, especially at the sites of subcellular organelles to trigger various cellular responses and control the cell fate. Each chapter of this thesis highlights the design and modification of the enzyme substrates for intracellular noncovalent synthesis of nanoarchitectures, as well as their potential bioapplications. Through the exploration of these applications, the work in the thesis aims to update the use of enzymatic reaction in regulating intermolecular interactions for targeting subcellular organelles and stimulate new developments in this simple and versatile approach for addressing societal needs.