Abstract
Following the observation of core circadian gene per expression in both Drosophila clock neurons and glial tissue in 1992, an ensuing vein of research has uncovered networks of circadian protein regulation and associated function.1 One instance found a structural and functional divergence between circadian architectures of glial cells and clock neurons per the glial expression of the cycling protein (and transcript of) Ebony.2 The extent of architectural divergence remains understudied, both between clock neurons and glia as well as between glial cell-types; absent is a comprehensive and corroborated circadian analysis of the glial transcriptome. In this work, I document a 2h, 12-timepoint single-cell RNA-seq transcriptome of adult Drosophila glia, I detail the cell-type specificity of circadian gene regulation, and I suggest corresponding regulatory mechanisms and functions. Notably, I evidence the comparative rhythmic prominence of ensheathing glia, a cell type relatively understudied in a circadian context. In addition, I cross-analyze results of four pertinent sequencing experiments and address two methodological flaws relevant to their limited reproducibility.