A Slow RNA Polymerase II Affects Alternative Splicing In Vivo

Manuel de la Mata; Claudio R Alonso; Sebastián Kadener; Juan P Fededa; Matı́as Blaustein; Federico Pelisch; Paula Cramer; David Bentley; Alberto R Kornblihtt

doi:10.1016/j.molcel.2003.08.001

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A Slow RNA Polymerase II Affects Alternative Splicing In Vivo

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A Slow RNA Polymerase II Affects Alternative Splicing In Vivo

Manuel de la Mata, Claudio R Alonso, Sebastián Kadener, Juan P Fededa, Matı́as Blaustein, Federico Pelisch, Paula Cramer, David Bentley and Alberto R Kornblihtt

Molecular Cell, Vol.12(2), pp.525-532

08/2003

DOI: https://doi.org/10.1016/j.molcel.2003.08.001

PMID: 14536091

Abstract

coupling

splicing

enzyme

RNA

Changes in promoter structure and occupation have been shown to modify the splicing pattern of several genes, evidencing a coupling between transcription and alternative splicing. It has been proposed that the promoter effect involves modulation of RNA pol II elongation rates. The C4 point mutation of the Drosophila pol II largest subunit confers on the enzyme a lower elongation rate. Here we show that expression of a human equivalent to Drosophila's C4 pol II in human cultured cells affects alternative splicing of the fibronectin EDI exon and adenovirus E1a pre-mRNA. Most importantly, resplicing of the Hox gene Ultrabithorax is stimulated in Drosophila embryos mutant for C4, which demonstrates the transcriptional control of alternative splicing on an endogenous gene. These results provide a direct proof for the elongation control of alternative splicing in vivo.

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Title: A Slow RNA Polymerase II Affects Alternative Splicing In Vivo
Creators: Manuel de la Mata - Laboratorio de Fisiologı́a y Biologı́a Molecular, Departamento de Fisiologı́a, Biologı́a Molecular y Celular, IFIBYNE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, (C1428EHA) Buenos Aires, Argentina
Claudio R Alonso - Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, United Kingdom
Sebastián Kadener - Laboratorio de Fisiologı́a y Biologı́a Molecular, Departamento de Fisiologı́a, Biologı́a Molecular y Celular, IFIBYNE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, (C1428EHA) Buenos Aires, Argentina
Juan P Fededa - Laboratorio de Fisiologı́a y Biologı́a Molecular, Departamento de Fisiologı́a, Biologı́a Molecular y Celular, IFIBYNE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, (C1428EHA) Buenos Aires, Argentina
Matı́as Blaustein - Laboratorio de Fisiologı́a y Biologı́a Molecular, Departamento de Fisiologı́a, Biologı́a Molecular y Celular, IFIBYNE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, (C1428EHA) Buenos Aires, Argentina
Federico Pelisch - Laboratorio de Fisiologı́a y Biologı́a Molecular, Departamento de Fisiologı́a, Biologı́a Molecular y Celular, IFIBYNE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, (C1428EHA) Buenos Aires, Argentina
Paula Cramer - Laboratorio de Fisiologı́a y Biologı́a Molecular, Departamento de Fisiologı́a, Biologı́a Molecular y Celular, IFIBYNE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, (C1428EHA) Buenos Aires, Argentina
David Bentley - Department of Biochemistry and Molecular Genetics, UCSC B121, 4200 East 9th Avenue, Denver, CO 80262 USA
Alberto R Kornblihtt - Laboratorio de Fisiologı́a y Biologı́a Molecular, Departamento de Fisiologı́a, Biologı́a Molecular y Celular, IFIBYNE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, (C1428EHA) Buenos Aires, Argentina
Publication Details: Molecular Cell, Vol.12(2), pp.525-532
Publisher: Elsevier Inc
Identifiers: 9923957819201921
Academic Unit: Rosenstiel Basic Medical Sciences Research Center; Department of Biology; Benjamin and Mae Volen National Center for Complex Systems; Interdepartmental Program in Neuroscience
Language: English
Resource Type: Journal article

A Slow RNA Polymerase II Affects Alternative Splicing In Vivo

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