Aberrant termination triggers nonsense-mediated mRNA decay

N Amrani; S Dong; F He; R Ganesan; S Ghosh; S Kervestin; C Li; D.A Mangus; P Spatrick; A Jacobson

doi:10.1042/BST0340039

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Journal article

Aberrant termination triggers nonsense-mediated mRNA decay

N Amrani, S Dong, F He, R Ganesan, S Ghosh, S Kervestin, C Li, D.A Mangus, P Spatrick and A Jacobson

Biochemical Society transactions, Vol.34(1), pp.39-42

02/01/2006

DOI: https://doi.org/10.1042/BST0340039

PMID: 16246174

Abstract

faux untranslated region

mRNA decay

translation termination

yeast

NMD (nonsense-mediated mRNA decay) is a cellular quality-control mechanism in which an otherwise stable mRNA is destabilized by the presence of a premature termination codon. We have defined the set of endogenous NMD substrates, demonstrated that they are available for NMD at every round of translation, and showed that premature termination and normal termination are not equivalent biochemical events. Premature termination is aberrant, and its NMD-stimulating defects can be reversed by the presence of tethered poly(A)-binding protein (Pab1p) or tethered eRF3 (eukaryotic release factor 3) (Sup35p). Thus NMD appears to be triggered by a ribosome's failure to terminate adjacent to a properly configured 3′-UTR (untranslated region), an event that may promote binding of the UPF/NMD factors to stimulate mRNA decapping.

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Title: Aberrant termination triggers nonsense-mediated mRNA decay
Creators: N Amrani - Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655-0122, U.S.A
S Dong - Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655-0122, U.S.A
F He - Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655-0122, U.S.A
R Ganesan - Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655-0122, U.S.A
S Ghosh - Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655-0122, U.S.A
S Kervestin - Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655-0122, U.S.A
C Li - Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655-0122, U.S.A
D.A Mangus - Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655-0122, U.S.A
P Spatrick - Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655-0122, U.S.A
A Jacobson - Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655-0122, U.S.A
Publication Details: Biochemical Society transactions, Vol.34(1), pp.39-42
Identifiers: 9923987246101921
Academic Unit: Department of Biology
Language: English
Resource Type: Journal article

Aberrant termination triggers nonsense-mediated mRNA decay

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