An RNAi-Based Approach Identifies Molecules Required for Glutamatergic and GABAergic Synapse Development

Suzanne Paradis; Dana B Harrar; Yingxi Lin; Alex C Koon; Jessica L Hauser; Eric C Griffith; Li Zhu; Lawrence F Brass; Chinfei Chen; Michael E Greenberg

doi:10.1016/j.neuron.2006.12.012

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An RNAi-Based Approach Identifies Molecules Required for Glutamatergic and GABAergic Synapse Development

Journal article

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An RNAi-Based Approach Identifies Molecules Required for Glutamatergic and GABAergic Synapse Development

Suzanne Paradis, Dana B Harrar, Yingxi Lin, Alex C Koon, Jessica L Hauser, Eric C Griffith, Li Zhu, Lawrence F Brass, Chinfei Chen and Michael E Greenberg

Neuron (Cambridge, Mass.), Vol.53(2), pp.217-232

2007

DOI: https://doi.org/10.1016/j.neuron.2006.12.012

PMID: 17224404

Abstract

RNA

MOLNEURO

SIGNALING

We report the results of a genetic screen to identify molecules important for synapse formation and/or maintenance. siRNAs were used to decrease the expression of candidate genes in neurons, and synapse development was assessed. We surveyed 22 cadherin family members and demonstrated distinct roles for cadherin-11 and cadherin-13 in synapse development. Our screen also revealed roles for the class 4 Semaphorins Sema4B and Sema4D in the development of glutamatergic and/or GABAergic synapses. We found that Sema4D affects the formation of GABAergic, but not glutamatergic, synapses. Our screen also identified the activity-regulated small GTPase Rem2 as a regulator of synapse development. A known calcium channel modulator, Rem2 may function as part of a homeostatic mechanism that controls synapse number. These experiments establish the feasibility of RNAi screens to characterize the mechanisms that control mammalian neuronal development and to identify components of the genetic program that regulate synapse formation and/or maintenance.

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Title: An RNAi-Based Approach Identifies Molecules Required for Glutamatergic and GABAergic Synapse Development
Creators: Suzanne Paradis - Neurobiology Program, Department of Neurology, Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA
Dana B Harrar - Neurobiology Program, Department of Neurology, Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA
Yingxi Lin - Neurobiology Program, Department of Neurology, Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA
Alex C Koon - Neurobiology Program, Department of Neurology, Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA
Jessica L Hauser - Neurobiology Program, Department of Neurology, Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA
Eric C Griffith - Neurobiology Program, Department of Neurology, Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA
Li Zhu - Department of Medicine, University of Pennsylvania, 421 Curie Boulevard, Philadelphia, PA 19104, USA
Lawrence F Brass - Department of Medicine, University of Pennsylvania, 421 Curie Boulevard, Philadelphia, PA 19104, USA
Chinfei Chen - Neurobiology Program, Department of Neurology, Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA
Michael E Greenberg - Neurobiology Program, Department of Neurology, Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA
Publication Details: Neuron (Cambridge, Mass.), Vol.53(2), pp.217-232
Publisher: Elsevier Inc
Identifiers: 9923958226501921
Academic Unit: Department of Biology; Benjamin and Mae Volen National Center for Complex Systems; Interdepartmental Program in Neuroscience
Language: English
Resource Type: Journal article

An RNAi-Based Approach Identifies Molecules Required for Glutamatergic and GABAergic Synapse Development

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