Bacterial killing via a type IV secretion system

Diorge P Souza; Gabriel U Oka; Cristina E Alvarez-Martinez; Alexandre W Bisson-Filho; German Dunger; Lise Hobeika; Nayara S Cavalcante; Marcos C Alegria; Leandro R S Barbosa; Roberto K Salinas; Cristiane R Guzzo; Chuck S Farah

doi:10.1038/ncomms7453

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Bacterial killing via a type IV secretion system

Journal article

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Bacterial killing via a type IV secretion system

Diorge P Souza, Gabriel U Oka, Cristina E Alvarez-Martinez, Alexandre W Bisson-Filho, German Dunger, Lise Hobeika, Nayara S Cavalcante, Marcos C Alegria, Leandro R S Barbosa, Roberto K Salinas, …

Nature communications, Vol.6(1), pp.6453-6453

03/06/2015

DOI: https://doi.org/10.1038/ncomms7453

PMID: 25743609

Abstract

Xanthomonas - metabolism

Escherichia coli

Crystallization

Models, Molecular

Scattering, Small Angle

Immunoblotting

Type IV Secretion Systems - chemistry

Bacteriolysis - physiology

X-Ray Diffraction

Cloning, Molecular

Antibiosis - physiology

Bacterial Proteins - metabolism

Protein Conformation

Bacterial Proteins - immunology

Microscopy, Fluorescence

Xanthomonas - physiology

Type IV Secretion Systems - metabolism

Immunoprecipitation

Type IV secretion systems (T4SSs) are multiprotein complexes that transport effector proteins and protein-DNA complexes through bacterial membranes to the extracellular milieu or directly into the cytoplasm of other cells. Many bacteria of the family Xanthomonadaceae, which occupy diverse environmental niches, carry a T4SS with unknown function but with several characteristics that distinguishes it from other T4SSs. Here we show that the Xanthomonas citri T4SS provides these cells the capacity to kill other Gram-negative bacterial species in a contact-dependent manner. The secretion of one type IV bacterial effector protein is shown to require a conserved C-terminal domain and its bacteriolytic activity is neutralized by a cognate immunity protein whose 3D structure is similar to peptidoglycan hydrolase inhibitors. This is the first demonstration of the involvement of a T4SS in bacterial killing and points to this special class of T4SS as a mediator of both antagonistic and cooperative interbacterial interactions.

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Title: Bacterial killing via a type IV secretion system
Creators: Diorge P Souza - Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, Sao Paulo, SP 05508-000, Brazil
Gabriel U Oka - Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, Sao Paulo, SP 05508-000, Brazil
Cristina E Alvarez-Martinez - 1] Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, Sao Paulo, SP 05508-000, Brazil Departamento de Genética, Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas, Rua Monteiro Lobato 255, Campinas, SP 13083-862, Brazil
Alexandre W Bisson-Filho - Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, Sao Paulo, SP 05508-000, Brazil
German Dunger - Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, Sao Paulo, SP 05508-000, Brazil
Lise Hobeika - Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, Sao Paulo, SP 05508-000, Brazil
Nayara S Cavalcante - Departamento de Física e Informática, Instituto de Física de São Carlos, Universidade de São Paulo, Avenida Trabalhador Sãocarlense 400, Sao Paulo, SP 13566-590, Brazil
Marcos C Alegria - Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, Sao Paulo, SP 05508-000, Brazil
Leandro R S Barbosa - Departamento de Física Geral, Instituto de Física, Universidade de São Paulo, Caixa Postal 66318, São Paulo, SP 05315-970, Brazil
Roberto K Salinas - Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, Sao Paulo, SP 05508-000, Brazil
Cristiane R Guzzo - 1] Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, Sao Paulo, SP 05508-000, Brazil Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes 1374, Sao Paulo, SP 05508-900, Brazil
Chuck S Farah - Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, Sao Paulo, SP 05508-000, Brazil
Publication Details: Nature communications, Vol.6(1), pp.6453-6453
Publisher: England
Identifiers: 9923977390801921
Academic Unit: Department of Biology
Language: English
Resource Type: Journal article

Bacterial killing via a type IV secretion system

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