Abstract
A procedure is described which extends the densitometric quantitation of phospholipids on ammonium sulfate impregnated thin-layer chromatograms by Gluck
et al. to include total lipid, free and esterified chlesterol, free fatty acid and triglyceride. Lipids separated on thin-layer plates containing silica gel G impregnated with ammonium sulfate were charred upon heating and absorbance was measured densitometrically. Thus, the necessity of spraying or submersing in a charring agent was eliminated, uniform charring became possible, and quantitation over a wider range of sample sizes than most densitometric procedures was obtained. One linear relationship existed for concentrations of standards over the range of 0.0–3.0 μg and another line from 4.0–50.0 μg. Both accuracy and precision of the method were highly reliable.