Abstract
Regulation of the
Drosophila
pigment-dispersing factor (
pdf
) gene products was analyzed in wild-type and clock mutants. Mutations in the transcription factors CLOCK and CYCLE severely diminish
pdf
RNA and neuropeptide (PDF) levels in a single cluster of clock-gene-expressing brain cells, called small ventrolateral neurons (
s-
LN
v
s). This clock-gene regulation of specific cells does not operate through an E-box found within
pdf
regulatory sequences. PDF immunoreactivity exhibits daily cycling, but only within terminals of axons projecting from the
s
-LN
v
s. This posttranslational rhythm is eliminated by
period
or
timeless
null mutations, which do not affect PDF staining in cell bodies or
pdf
mRNA levels. Therefore, within these chronobiologically important neurons, separate elements of the central pacemaking machinery regulate
pdf
or its product in novel and different ways. Coupled with contemporary results showing a
pdf-
null mutant to be severely defective in its behavioral rhythmicity, the present results reveal PDF as an important circadian mediator whose expression and function are downstream of the clockworks.