Dynamic profiling of the protein life cycle in response to pathogens

Marko Jovanovic; Michael S Rooney; Philipp Mertins; Dariusz Przybylski; Nicolas Chevrier; Rahul Satija; Edwin H Rodriguez; Alexander P Fields; Schraga Schwartz; Raktima Raychowdhury; Maxwell R Mumbach; Thomas Eisenhaure; Michal Rabani; Dave Gennert; Diana Lu; Toni Delorey; Jonathan S Weissman; Steven A Carr; Nir Hacohen; Aviv Regev

doi:10.1126/science.1259038

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Dynamic profiling of the protein life cycle in response to pathogens

Journal article

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Dynamic profiling of the protein life cycle in response to pathogens

Marko Jovanovic, Michael S Rooney, Philipp Mertins, Dariusz Przybylski, Nicolas Chevrier, Rahul Satija, Edwin H Rodriguez, Alexander P Fields, Schraga Schwartz, Raktima Raychowdhury, …

Science (American Association for the Advancement of Science), Vol.347(6226), pp.1259038-1259038

03/06/2015

DOI: https://doi.org/10.1126/science.1259038

PMCID: PMC4506746

PMID: 25745177

Abstract

Protein expression is regulated by production and degradation of mRNAs and proteins, but their specific relationships remain unknown. We combine measurements of protein production and degradation and mRNA dynamics to build a quantitative genomic model of the differential regulation of gene expression in LPS-stimulated mouse dendritic cells. Changes in mRNA abundance play a dominant role in determining most dynamic fold changes in protein levels. Conversely, the preexisting proteome of proteins performing basic cellular functions is remodeled primarily through changes in protein production or degradation, accounting for over half of the absolute change in protein molecules in the cell. Thus, the proteome is regulated by transcriptional induction of novel cellular functions and remodeling of preexisting functions through the protein life cycle.

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Title: Dynamic profiling of the protein life cycle in response to pathogens
Creators: Marko Jovanovic - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Michael S Rooney - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Philipp Mertins - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Dariusz Przybylski - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Nicolas Chevrier - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Rahul Satija - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Edwin H Rodriguez - Department of Cellular and Molecular Pharmacology, California Institute for Quantitative Biomedical Research, University of California, San Francisco, San Francisco, CA 94158
Alexander P Fields - Department of Cellular and Molecular Pharmacology, California Institute for Quantitative Biomedical Research, University of California, San Francisco, San Francisco, CA 94158
Schraga Schwartz - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Raktima Raychowdhury - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Maxwell R Mumbach - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Thomas Eisenhaure - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Michal Rabani - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Dave Gennert - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Diana Lu - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Toni Delorey - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Jonathan S Weissman - Department of Cellular and Molecular Pharmacology, California Institute for Quantitative Biomedical Research, University of California, San Francisco, San Francisco, CA 94158
Steven A Carr - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Nir Hacohen - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Aviv Regev - Broad Institute of MIT and Harvard, Cambridge, MA 02142
Publication Details: Science (American Association for the Advancement of Science), Vol.347(6226), pp.1259038-1259038
Identifiers: 9924012913901921
Academic Unit: Department of Biology
Language: English
Resource Type: Journal article

Dynamic profiling of the protein life cycle in response to pathogens

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