Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99–125

Zheng LI; Li DENG; Jike LU; Xiaolei GUO; Zixin YANG; Tianwei TAN

doi:10.1016/S1004-9541(09)60141-5

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Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99–125

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Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99–125

Zheng LI, Li DENG, Jike LU, Xiaolei GUO, Zixin YANG and Tianwei TAN

Chinese journal of chemical engineering, Vol.18(5), pp.870-875

10/2010

DOI: https://doi.org/10.1016/S1004-9541(09)60141-5

Abstract

rice bran oil

methanolysis

transesterification

immobilized lipase

Optimization

The non-edible crude rice bran oil was extracted from white rice bran, and then was catalyzed by immobilized lipase for biodiesel production in this study. The effects of water content, oil/methanol molar ratio, temperature, enzyme amount, solvent, number of methanol added times and two-step methanolysis by using Candida sp. 99-125 as catalyst were investigated. The optimal conditions for processing 1 g rice bran oil were: 0.2 g immobilized lipase, 2 ml n-hexane as solvent, 20% water based on the rice bran oil mass, temperature of 40 °C and two-step addition of methanol. As a result, the fatty acid methyl esters yield was 87.4%. The immobilized lipase was proved to be stable when it was used repeatedly for 7 cycles.

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Title: Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99–125
Creators: Zheng LI - Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
Li DENG - Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
Jike LU - Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
Xiaolei GUO - Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
Zixin YANG - Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
Tianwei TAN - Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
Publication Details: Chinese journal of chemical engineering, Vol.18(5), pp.870-875
Publisher: Elsevier B.V
Grant note: (2006AA020101, 2007AA10Z360, 2009AA03Z232) / the National High Technology Research and Development Program of China (2008BA163B07) / Key Projects in the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period
Identifiers: 9923972895601921
Academic Unit: Department of Chemistry
Language: English
Resource Type: Journal article

Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99–125

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