Abstract
Osteoblasts isolated from the cranium of Zelanian rabbit, after being transfected with green fluorescent protein, were inoculated onto the bio-derived bone to investigate the 3D fabrication of engineering bone in rotating wall vessel bioreactor (RWVB). As a control, the cell-scaffold constructs were cultured in T-flasks simultaneously. During one week's culture, the samples were collected every 12 h. The histological and immunohistochemical assays were operated respectively. Furthermore, the cells' metabolism of nutrients was monitored and analyzed during the whole culture process. The results show that the cell number fabricated in RWVB was five times of that in T-flasks. Bone tissue fabricated in RWVB with two different densities grew well, and osteoblasts maintained normal chromosome. It is demonstrated that with the stress stimulation inside the fluid in RWVB, the active expression of ALP can be increased, a rapid proliferation and differentiation of osteoblasts are possible and the 3D fabrication of engineering bone could be realized.