Abstract
Work on sleep at Brandeis focuses on Drosophila melanogaster as well as the more traditional rodent models. The Drosophila works aims to exploit the genetic advantages of this organism yet still learn about aspcts os sleep relevant to humans. The major finding has been that the human therapeutic carbamazepine is a potent sleep-deprivation agent inflies. Current data indicate that its effects are mediated through the rdl GABA receptor, which has implications for the role of this drug in humans. One of the rodent laboratories is focused on the regulation of sleep and waking in the basal forebrain. The goal is to identify gene expression changes in its cholinergic neuronal subset, and specific neuron purification has been accomplished. Another rodent laboratory is tudying the effects of sleep deprivation on the intrinsic electrophysiology and gene expression properties of neocortical neurons. Interesting changes in firing properties of layer 5 pyramidal neurons has been observed, and gene expression assays from these cells are underway. The final two projects involve the role of sleep in homeostatic plasticity and fear conditioning. These are being done both in vivo, in freely behaving animals, and ex vivo, in cortical slices after sleep deprivation or training.