Identification of a cysteine residue as the binding site for the dipyrromethane cofactor at the active site of Escherichia coli porphobilinogen deaminase

Peter M Jordan; Martin J Warren; Howard J Williams; Neal J Stolowich; Charles A Roessner; Stephen K Grant; A.Ian Scott

doi:10.1016/0014-5793(88)81260-2

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Identification of a cysteine residue as the binding site for the dipyrromethane cofactor at the active site of Escherichia coli porphobilinogen deaminase

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Identification of a cysteine residue as the binding site for the dipyrromethane cofactor at the active site of Escherichia coli porphobilinogen deaminase

Peter M Jordan, Martin J Warren, Howard J Williams, Neal J Stolowich, Charles A Roessner, Stephen K Grant and A.Ian Scott

FEBS letters, Vol.235(1-2), pp.189-193

08/01/1988

DOI: https://doi.org/10.1016/0014-5793(88)81260-2

PMID: 3042456

Abstract

13C-NMR

Cysteine

Dipyrromethane cofactor

Porphobilinogen deaminase

E. coli

The dipyrromethane cofactor of Escherichia coli porphobilinogen deaminase was specifically labelled with 13C by growth of the bacteria in the presence of 5-amino[5-13C]levulinic acid. Using 13C-NMR spectroscopy, the structure of the cofactor was confirmed as a dipyrromethane made up of two linked pyrrole rings each derived from porphobilinogen. The chemical shift data indicate that one of the pyrrole rings of the cofactor is covalently linked to the deaminase enzyme through a cysteine residue. Evidence from protein chemistry studies suggest that cysteine-242 is the covalent binding site for the cofactor.

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Title: Identification of a cysteine residue as the binding site for the dipyrromethane cofactor at the active site of Escherichia coli porphobilinogen deaminase
Creators: Peter M Jordan - Department of Biochemistry, University of Southampton, Southampton SO9 3TU, England
Martin J Warren - Department of Biochemistry, University of Southampton, Southampton SO9 3TU, England
Howard J Williams - Department of Chemistry, Texas A&M University, College Station, TX 77843-3255, USA
Neal J Stolowich - Department of Chemistry, Texas A&M University, College Station, TX 77843-3255, USA
Charles A Roessner - Department of Chemistry, Texas A&M University, College Station, TX 77843-3255, USA
Stephen K Grant - Department of Chemistry, Texas A&M University, College Station, TX 77843-3255, USA
A.Ian Scott - Department of Chemistry, Texas A&M University, College Station, TX 77843-3255, USA
Publication Details: FEBS letters, Vol.235(1-2), pp.189-193
Publisher: Elsevier B.V
Identifiers: 9924012519901921
Academic Unit: Department of Chemistry
Language: English
Resource Type: Journal article

Identification of a cysteine residue as the binding site for the dipyrromethane cofactor at the active site of Escherichia coli porphobilinogen deaminase

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