Abstract
To investigate the kinetics of the prolyl peptide bond cis/trans isomerization of /V-succinyl- Ala-Phe-Pro-Phe-(4-)nitroanilide catalyzed by peptidyl prolyl cis/trans isomerases (PPIases), one- dimensional dynamic 1 **H NMR spectroscopy was employed. To this end line shape analyses of proton
signals were performed at various concentrations of both cytosolic porcine kidney cyclophilin (CyplS) and peptide substrate. Catalysis of the cis/trans isomerization by CyplS is best described by a four-site exchange model, where the four sites represent the cis and trans isomers free in solution and bound to the enzyme. Combination of dynamic NMR spectroscopy with the classical protease-coupled PPIase assay allowed determination of the complete set of the microscopic rate constants describing the four site exchange model. The comparison of the rate constants of cis — trans isomerization of the peptide free in solution and bound to cyclophilin yields an acceleration factor of 3.5 x 105 **8. Dissociation of the Michaelis complexes are of the same order of magnitude as the isomerization rates on the enzyme. Therefore, all microscopic rate constants contribute to the steady state parameters. For the first time, the kcat (620 s_I) and Km (220 μ ) value for the trans isomer in addition to the values of the cis isomer (fccat = 680 s-1, Km = 80 μ ) could be determined under reversible conditions at pH 6.0 and 10 °C. The affinity of CyplS for the cis isomer is 4 times higher than for the trans isomer. This results in a shift of the cis/trans equilibrium toward the cis isomer. The 1.8 ppm downfield chemical shift of the cis signal of the substrate amide
proton of Phe5 could be explained by hydrogen bonding of the cis peptide unit to the enzyme. This might be the reason for the preferred binding of the cis isomer. Finally, the results suggest that during catalysis the residues N-terminal to proline remain fixed to CyplS while the C-terminal part is rotated.