Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.
- Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
- Joshua A Welsh - Translational Nanobiology Section, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USADeborah C I Goberdhan - Nuffield Department of Women's and Reproductive Health, University of Oxford, Women's Centre, John Radcliffe Hospital, Oxford, UKLorraine O'Driscoll - Trinity College DublinEdit I Buzas - HUN-REN-SU Translational Extracellular Vesicle Research Group, Semmelweis University, Budapest, HungaryCherie Blenkiron - Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New ZealandBenedetta Bussolati - Department of Molecular Biotechnology and Health Sciences, University of Turin, Turin, ItalyHoujian Cai - University of Georgia, Athens, Georgia, USADolores Di Vizio - Department of Surgery, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, California, USATom A P Driedonks - Department CDL Research, University Medical Center Utrecht, Utrecht, The NetherlandsUta Erdbrügger - University of Virginia Health System, Charlottesville, Virginia, USAJuan M Falcon-Perez - IKERBASQUE, Basque Foundation for Science, Bilbao, SpainQing-Ling Fu - Extracellular Vesicle Research and Clinical Translational Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaAndrew F Hill - Institute for Health and Sport, Victoria University, Melbourne, AustraliaMetka Lenassi - Faculty of Medicine, University of Ljubljana, Ljubljana, SloveniaSai Kiang Lim - Department of Surgery, YLL School of Medicine, National University Singapore, Singapore, SingaporeMỹ G Mahoney - Thomas Jefferson UniversitySujata Mohanty - Stem Cell Facility, All India Institute of Medical Sciences, New Delhi, IndiaAndreas Möller - QIMR Berghofer Medical Research Institute, Brisbane, AustraliaRienk Nieuwland - Amsterdam Vesicle Center, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, Amsterdam, The NetherlandsTakahiro Ochiya - Tokyo Medical University, Tokyo, JapanSusmita Sahoo - Icahn School of Medicine at Mount Sinai, New York, New York, USAAna C Torrecilhas - Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários, Departamento de Ciências Farmacêuticas, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo (UNIFESP) Campus Diadema, Diadema, BrazilLei Zheng - Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaAndries Zijlstra - Genentech, South San Francisco, California, USASarah Abuelreich - Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USAReem Bagabas - Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USAPaolo Bergese - National Center for Gene Therapy and Drugs based on RNA Technology, Padua, ItalyEsther M Bridges - Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UKMarco Brucale - Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase, Florence, ItalyDylan Burger - School of Pharmaceutical Sciences, University of Ottawa, Ottawa, CanadaRandy P Carney - Department of Biomedical Engineering, University of California, Davis, California, USAEmanuele Cocucci - Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio, USARossella Crescitelli - Wallenberg Centre for Molecular and Translational Medicine, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenEdveena Hanser - Department of Biomedicine, University of Basel, Basel, SwitzerlandAdrian L Harris - Department of Oncology, University of Oxford, Oxford, UKNorman J Haughey - Departments of Neurology and Psychiatry, Johns Hopkins University School of Medicine, Baltimore, Maryland, USAAn Hendrix - Cancer Research Institute Ghent, Ghent, BelgiumAlexander R Ivanov - Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USATijana Jovanovic-Talisman - Department of Cancer Biology and Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USANicole A Kruh-Garcia - Bio-pharmaceutical Manufacturing and Academic Resource Center (BioMARC), Infectious Disease Research Center, Colorado State University, Fort Collins, Colorado, USAVroniqa Ku'ulei-Lyn Faustino - Beckman Research InstituteDiego Kyburz - Department of Rheumatology, University Hospital Basel, Basel, SwitzerlandCecilia Lässer - Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine at Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenKathleen M Lennon - Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USAJan Lötvall - Krefting Research Centre, Institute of Medicine at Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenAdam L Maddox - Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USAElena S Martens-Uzunova - Erasmus MC Cancer Institute, University Medical Center Rotterdam, Department of Urology, Rotterdam, The NetherlandsRachel R Mizenko - Department of Biomedical Engineering, University of California, Davis, California, USALauren A Newman - College of Medicine and Public Health, Flinders University, Adelaide, AustraliaAndrea Ridolfi - Department of Physics and Astronomy, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam, Amsterdam, The NetherlandsEva Rohde - Transfer Centre for Extracellular Vesicle Theralytic Technologies, EV-TT, Salzburg, AustriaTatu Rojalin - Expansion Therapeutics, Structural Biology and Biophysics, Jupiter, Florida, USAAndrew Rowland - College of Medicine and Public Health, Flinders University, Adelaide, AustraliaAndras Saftics - Department of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USAUrsula S Sandau - Department of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USAJulie A Saugstad - Department of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USAFaezeh Shekari - Celer Diagnostics, Toronto, CanadaSimon Swift - Waipapa Taumata Rau University of Auckland, Auckland, New ZealandDmitry Ter-Ovanesyan - Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USAJuan P Tosar - Institut Pasteur de Montevideo, Montevideo, UruguayZivile Useckaite - College of Medicine and Public Health, Flinders University, Adelaide, AustraliaFrancesco Valle - Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase, Florence, ItalyZoltan Varga - Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, HungaryEdwin van der Pol - Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, location AMC, University of Amsterdam, Amsterdam, The NetherlandsMartijn J C van Herwijnen - Utrecht UniversityMarca H M Wauben - Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The NetherlandsAnn M Wehman - University of Denver, Denver, USASarah Williams - International Society for Extracellular VesiclesAndrea Zendrini - Center for Colloid and Surface Science (CSGI), Florence, ItalyAlan J Zimmerman - Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USAClotilde Théry - CurieCoreTech Extracellular Vesicles, Institut Curie, Paris, FranceKenneth W Witwer - The Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease, Johns Hopkins University School of Medicine, Baltimore, Maryland, USAAvital Rodal - Brandeis University, Department of BiologyMISEV Consortium
- Journal of extracellular vesicles, Vol.13(2), pp.e12404-n/a
- L30 MH131119 / NIMH NIH HHS MR/R025096/1 / Medical Research Council R01 DK136511 / NIDDK NIH HHS P01 CA229123 / NCI NIH HHS R01 EY034193 / NEI NIH HHS R56 EY034193 / NEI NIH HHS P01 CA019014 / NCI NIH HHS R01 CA249684 / NCI NIH HHS R01 CA206458 / NCI NIH HHS P20 GM152335 / NIGMS NIH HHS
- 9924391887001921
- Benjamin and Mae Volen National Center for Complex Systems; Department of Biology; Rosenstiel Basic Medical Sciences Research Center; Interdepartmental Program in Neuroscience; School of Arts and Sciences
- English
- Journal article