Modification of a DNA cloning vehicle to give a high strand-separation melting temperature

Raymond L White; Michael Rosbach

doi:10.1016/0378-1119(79)90026-X

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Modification of a DNA cloning vehicle to give a high strand-separation melting temperature

Journal article

Peer reviewed

Modification of a DNA cloning vehicle to give a high strand-separation melting temperature

Raymond L White and Michael Rosbach

Gene, Vol.7(2), pp.97-107

10/1979

DOI: https://doi.org/10.1016/0378-1119(79)90026-X

PMID: 116908

Abstract

electron microscopy of R-loops

high G+C vector

Recombinant DNA

We have constructed a recombinant DNA cloning vector with a high strand-separation melting temperature. This feature is the result of an insertion of a short sequence of dG:dC base pairs. The new vector is useful for cloning DNA segments in anticipation of forming R-loops for the purpose of either transcriptional mapping or gene isolation.

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Title: Modification of a DNA cloning vehicle to give a high strand-separation melting temperature
Creators: Raymond L White - Department of Microbiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Michael Rosbach - Rosenstiel Basic Medical Sciences Research Center and Department of Biology, Brandeis University, Waltham, MA 02154 U.S.A
Publication Details: Gene, Vol.7(2), pp.97-107
Publisher: Elsevier B.V
Identifiers: 9923958134601921
Language: English
Resource Type: Journal article

Modification of a DNA cloning vehicle to give a high strand-separation melting temperature

Abstract

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