Multivalent glycocluster design through directed evolution

Iain S MacPherson; J. Sebastian Temme; Sevan Habeshian; Krzysztof Felczak; Krzysztof Pankiewicz; Lizbeth Hedstrom; Isaac J Krauss

doi:10.1002/anie.201105555

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Multivalent glycocluster design through directed evolution

Journal article

Peer reviewed

Multivalent glycocluster design through directed evolution

Iain S MacPherson, J. Sebastian Temme, Sevan Habeshian, Krzysztof Felczak, Krzysztof Pankiewicz, Lizbeth Hedstrom and Isaac J Krauss

Angewandte Chemie (International ed.), Vol.50(47), pp.11238-11242

11/18/2011

DOI: https://doi.org/10.1002/anie.201105555

PMID: 22191092

Abstract

Directed Evolution

2G12

Carbohydrate Cluster Effect

Multivalency

Glycoconjugates

Antibodies

Biochemistry

Carbohydrates

DNA

Selection with modified aptamers: A method is described for design of glycocluster ligands by directed evolution. Glycan azides are attached to a library of alkyne-containing DNA sequences. DNA sequences which cluster the glycans best for multivalent binding to the target are selected and amplified. This method has been applied to the development of high-mannose clusters which bind HIV-neutralizing antibody 2G12.

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Title: Multivalent glycocluster design through directed evolution
Creators: Iain S MacPherson (Author) - Department of Chemistry Brandeis University 415 South St. Waltham, MA 02454
J. Sebastian Temme (Author) - Department of Chemistry Brandeis University 415 South St. Waltham, MA 02454
Sevan Habeshian (Author) - Department of Chemistry Brandeis University 415 South St. Waltham, MA 02454
Krzysztof Felczak (Author) - Center for Drug Design University of Minnesota 516 Delaware St. SE MMC 204 Minneapolis, MN 55455
Krzysztof Pankiewicz (Author) - Center for Drug Design University of Minnesota 516 Delaware St. SE MMC 204 Minneapolis, MN 55455
Lizbeth Hedstrom (Author) - Departments of Biology and Chemistry Brandeis University 415 South St. Waltham, MA 02454
Isaac J Krauss (Author) - Department of Chemistry Brandeis University 415 South St. Waltham, MA 02454
Publication Details: Angewandte Chemie (International ed.), Vol.50(47), pp.11238-11242
Identifiers: 9923958417601921
Academic Unit: Department of Chemistry; Department of Biology
Language: English
Resource Type: Journal article

Multivalent glycocluster design through directed evolution

Abstract

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