Abstract
Homothallic strains of Saccharomyces cerevisiae can switch from one mating type to the other as often as every cell division. The conversion of mating type alleles (from MATa to MATα or vice versa) depends on other, unexpressed copies of a or α information that can be transposed to MAT. Previously, “inconvertible” mutations within MATα and MATa have been described that block the excision of the MAT allele. In this paper we describe two cis-acting mutations that also impair mating type switching and lie very near, but outside, the MAT locus. Both “stuck” mutations, stk1 and stk2, diminish the efficiency of converting MATa to MATα to less than 10% of normal. The stk1 mutation also slightly reduces conversion of MATα to MATa, whereas stk2 has no discernible effect. Unlike the inconvertible MATα-inc and MATa-inc mutations within MAT, the stk mutations are not replaced by wild-type sequences after the “stuck” cells occasionally switch to the opposite mating type. Because these mutations are not “healed” by mating type conversions, they must lie in sequences outside of the transposable mating type information. These results indicate that the efficient replacement of MAT alleles depends on sequences both within and adjacent to the MAT locus. Among subclones of homothallic stk MATa strains, approximately 2% show “illegal” transpositions of mating type genes. In these colonies the silent copy of α information at the HMLα locus has been converted to a, without any change of MATa or the silent a copy at HMRa. Such conversions of the unexpressed library genes are not found in wild-type homothallic strains that can switch mating type efficiently, but they are found in MATa-inc and MATα-inc strains. It appears that all of the cis-acting mutations within or adjacent to mating type result in these unusual switches of mating type information at HML and HMR.