Nitrilotriacetic Acid-Modified Magnetic Nanoparticles as a General Agent to Bind Histidine-Tagged Proteins

Chenjie XU; Keming XU; Hongwei GU; Xiaofen Zhong; Zhihong Guo; Rongkun Zheng; Xixiang Zhang; Bing XU

doi:10.1021/ja031776d

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Nitrilotriacetic Acid-Modified Magnetic Nanoparticles as a General Agent to Bind Histidine-Tagged Proteins

Journal article

Peer reviewed

Nitrilotriacetic Acid-Modified Magnetic Nanoparticles as a General Agent to Bind Histidine-Tagged Proteins

Chenjie XU, Keming XU, Hongwei GU, Xiaofen Zhong, Zhihong Guo, Rongkun Zheng, Xixiang Zhang and Bing XU

Journal of the American Chemical Society, Vol.126(11), pp.3392-3393

03/01/2004

DOI: https://doi.org/10.1021/ja031776d

PMID: 15025444

Abstract

Solution chemistry

Nanoparticles

Ions

Imidazoles

Imaging probes

Using Nα,Nα-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt−NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.

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Title: Nitrilotriacetic Acid-Modified Magnetic Nanoparticles as a General Agent to Bind Histidine-Tagged Proteins
Creators: Chenjie XU - Department of Chemistry, Department of Physics, and Bioengineering Program,The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
Keming XU - Department of Chemistry, Department of Physics, and Bioengineering Program,The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
Hongwei GU - Department of Chemistry, Department of Physics, and Bioengineering Program,The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
Xiaofen Zhong - Department of Chemistry, Department of Physics, and Bioengineering Program,The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
Zhihong Guo - Department of Chemistry, Department of Physics, and Bioengineering Program,The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
Rongkun Zheng - Department of Chemistry, Department of Physics, and Bioengineering Program,The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
Xixiang Zhang - Department of Chemistry, Department of Physics, and Bioengineering Program,The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
Bing XU - Department of Chemistry, Department of Physics, and Bioengineering Program,The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
Publication Details: Journal of the American Chemical Society, Vol.126(11), pp.3392-3393
Publisher: American Chemical Society
Identifiers: 9924013284001921
Academic Unit: Department of Chemistry
Language: English
Resource Type: Journal article

Nitrilotriacetic Acid-Modified Magnetic Nanoparticles as a General Agent to Bind Histidine-Tagged Proteins

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