Abstract
The response surface methodology was employed to optimize the integrated extraction parameters of cordycepic acid and cordycepinfrom cultured Cordyceps militaris (L.) link based on a single-factor experiment. The Box-Behnken design with three independentvariablesi.e., microwave power (W), water/material ratio (mL/g) and extraction time (min) was used. The experimental data obtained were fitted toa second-order polynomial equation using multiple regression analysis. The three-dimensional response surface plot and contour plotderiv ed from the mathematical models were applied to determine the optimal conditions. The optimum extraction condition was obtainedas follows: microwave-assisted extraction, microwave power of 649.33 W, extraction time of 5.74 min, water/material ratio of 38. 99 mL/gand extraction number of three. The yield of cordycepic acid and cordycepin were 2.47 and 0.79 %, respectively. Under these conditions,the experimental values of 3.12 and 0.75 % well agr eed with those predicted by the model.
