PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator

Steven A BROWN; Juergen RIPPERGER; Sebastian KADENER; Fabienne FLEURY-OLELA; Francis VITBOIS; Michael ROSBASH; Ueli SCHIBLER

doi:10.1126/science.1107373

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PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator

Journal article

Peer reviewed

PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator

Steven A BROWN, Juergen RIPPERGER, Sebastian KADENER, Fabienne FLEURY-OLELA, Francis VITBOIS, Michael ROSBASH and Ueli SCHIBLER

Science (American Association for the Advancement of Science), Vol.308(5722), pp.693-696

04/29/2005

DOI: https://doi.org/10.1126/science.1107373

PMID: 15860628

Abstract

Biological and medical sciences

Chronobiology

Fundamental and applied biological sciences

vertebrates

oscillator

Psychology

Anatomy

The clock proteins PERIOD1 (PER1) and PERIOD2 (PER2) play essential roles in a negative transcriptional feedback loop that generates circadian rhythms in mammalian cells. We identified two PER1-associated factors, NONO and WDR5, that modulate PER activity. The reduction of NONO expression by RNA interference (RNAi) attenuated circadian rhythms in mammalian cells, and fruit flies carrying a hypomorphic allele were nearly arrhythmic. WDR5, a subunit of histone methyltransferase complexes, augmented PER-mediated transcriptional repression, and its reduction by RNAi diminished circadian histone methylations at the promoter of a clock gene.

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Title: PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator
Creators: Steven A BROWN - Department of Molecular Biology and National Centres of Competence in Research (NCCR) Frontiers in Genetics, Sciences III, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva, Switzerland
Juergen RIPPERGER - Department of Molecular Biology and National Centres of Competence in Research (NCCR) Frontiers in Genetics, Sciences III, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva, Switzerland
Sebastian KADENER - Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454, United States
Fabienne FLEURY-OLELA - Department of Molecular Biology and National Centres of Competence in Research (NCCR) Frontiers in Genetics, Sciences III, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva, Switzerland
Francis VITBOIS - Serono Pharmaceutical Research Institute, 14 Chemin des Aulx, 1228 Plan-les-Ouates, Geneva, Switzerland
Michael ROSBASH - Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454, United States
Ueli SCHIBLER - Department of Molecular Biology and National Centres of Competence in Research (NCCR) Frontiers in Genetics, Sciences III, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva, Switzerland
Publication Details: Science (American Association for the Advancement of Science), Vol.308(5722), pp.693-696
Publisher: American Association for the Advancement of Science; Washington, DC
Identifiers: 9923958142401921
Academic Unit: Rosenstiel Basic Medical Sciences Research Center; Department of Biology; Benjamin and Mae Volen National Center for Complex Systems; Interdepartmental Program in Neuroscience
Language: English
Resource Type: Journal article

PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator

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