Abstract
Without the
RAD51
strand exchange protein,
Saccharomyces cerevisiae
cannot repair a double-strand break (DSB) by gene conversion. However, cells can repair DSBs by recombination-dependent, break-induced replication (BIR).
RAD51
-independent BIR is initiated more than 13 kb from the DSB. Repair depends on a 200-bp sequence adjacent to
ARS310
, located ∼34 kb centromere-proximal to the DSB, but does not depend on the origin activity of
ARS310
. We conclude that the ability of a recombination-induced replication fork to copy >130 kb to the end of the chromosome depends on a special site that enhances assembly of a processive repair replication fork.