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SEIRA Spectroscopy of the Electrochemical Activation of an Immobilized [NiFe] Hydrogenase under Turnover and Non-Turnover Conditions
Journal article   Peer reviewed

SEIRA Spectroscopy of the Electrochemical Activation of an Immobilized [NiFe] Hydrogenase under Turnover and Non-Turnover Conditions

Diego Millo, Peter Hildebrandt, Maria-Eirini Pandelia, Wolfgang Lubitz and Ingo Zebger
Angewandte Chemie (International ed.), Vol.50(11), pp.2632-2634
03/07/2011
DOI: https://doi.org/10.1002/anie.201006646
PMID: 21370354

Abstract

SEIRA spectroscopy hydrogenases biocatalysis redox titration Electrochemistry
Effective and unaffected: An [NiFe] hydrogenase was immobilized on Au electrodes. Surface-enhanced infrared absorption (SEIRA) spectroscopy monitored structural changes of the active site as a function of the applied potential. The redox titration of the enzyme gave a formal potential (E1/2) similar to that obtained for the enzyme in solution, demonstrating that the structure of the active site is not affected by the immobilization.

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