Single-molecule force spectroscopy and imaging of the vancomycin/D-Ala-D-Ala interaction

Yann Gilbert; Marie Deghorain; Pascal Hols; Yves F Dufrene; LING Wang; BING XU; Philipp D Pollheimer; Hermann J Gruber; Jeff Errington; Bernard Hallet; Xavier Haulot; Claire Verbelen

doi:10.1021/nl0700853

Back

Single-molecule force spectroscopy and imaging of the vancomycin/D-Ala-D-Ala interaction

Journal article

Peer reviewed

Single-molecule force spectroscopy and imaging of the vancomycin/D-Ala-D-Ala interaction

Yann Gilbert, Marie Deghorain, Pascal Hols, Yves F Dufrene, LING Wang, BING XU, Philipp D Pollheimer, Hermann J Gruber, Jeff Errington, Bernard Hallet, …

Nano letters, Vol.7(3), pp.796-801

03/01/2007

DOI: https://doi.org/10.1021/nl0700853

PMID: 17316058

Abstract

Fundamental and applied biological sciences. Psychology

Others

Biological and medical sciences

Methods. Procedures. Technologies

Various methods and equipments

Biotechnology

The clinically important vancomycin antibiotic inhibits the growth of pathogens such as Staphylococcus aureus by blocking cell wall synthesis through specific recognition of nascent peptidoglycan terminating in d-Ala-d-Ala. Here, we demonstrate the ability of single-molecule atomic force microscopy with antibiotic-modified tips to measure the specific binding forces of vancomycin and to map individual ligands on living bacteria. The single-molecule approach presented here provides new opportunities for understanding the binding mechanisms of antibiotics and for exploring the architecture of bacterial cell walls.

Metrics

18 Record Views

See more details

Details

Title: Single-molecule force spectroscopy and imaging of the vancomycin/D-Ala-D-Ala interaction
Creators: Yann Gilbert - Unité de Chimie des Interfaces, Université catholique de Louvain, Croix du Sud 2/18, 1348 Louvain-la-Neuve, Belgium
Marie Deghorain - Unité de Génétique, Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 5/6, 1348 Louvain-la-Neuve, Belgium
Pascal Hols - Unité de Chimie des Interfaces, Université catholique de Louvain, Croix du Sud 2/18, 1348 Louvain-la-Neuve, Belgium
Yves F Dufrene - Unité de Chimie des Interfaces, Université catholique de Louvain, Croix du Sud 2/18, 1348 Louvain-la-Neuve, Belgium
LING Wang - Department of Chemistry, Hong Kong University of Science & Technology, Hong-Kong
BING XU - Department of Chemistry, Hong Kong University of Science & Technology, Hong-Kong
Philipp D Pollheimer - Institute for Biophysics, Johannes Kepler University of Linz, Altenbergerstr.69, 4040 Linz, Austria
Hermann J Gruber - Institute for Biophysics, Johannes Kepler University of Linz, Altenbergerstr.69, 4040 Linz, Austria
Jeff Errington - Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle NE2 4HH, United Kingdom
Bernard Hallet - Unité de Génétique, Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 5/6, 1348 Louvain-la-Neuve, Belgium
Xavier Haulot - Unité de Chimie des Interfaces, Université catholique de Louvain, Croix du Sud 2/18, 1348 Louvain-la-Neuve, Belgium
Claire Verbelen - Unité de Chimie des Interfaces, Université catholique de Louvain, Croix du Sud 2/18, 1348 Louvain-la-Neuve, Belgium
Publication Details: Nano letters, Vol.7(3), pp.796-801
Publisher: American Chemical Society
Identifiers: 9924013272601921
Academic Unit: Department of Chemistry
Language: English
Resource Type: Journal article

Single-molecule force spectroscopy and imaging of the vancomycin/D-Ala-D-Ala interaction

Abstract

Metrics

Details

Brandeis University Social media