Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination

Andrés Aguilera; Gregory Ira; Audrey Ceschia; Stefan Uhle; Violeta Cordon-Preciado; Ji-Young Hwang; Andrés Clemente-Blanco; Pranav Ullal; Jordi Torres-Rosell; Sarah Farmer; Beatriz Leitao; Kyungjae Myung; Alexandra McAleenan; Luis Aragón; Felipe Cortes-Ledesma; Debra Bressan; Alma Papusha; Farokh Dotiwala; Felix Machin; Adam Jarmuz; Giacomo De Piccoli; Felip Vilella-Mitjana; Xiaolan Zhao; James E Haber

doi:10.1038/ncb1466

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Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination

Journal article

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Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination

Andrés Aguilera, Gregory Ira, Audrey Ceschia, Stefan Uhle, Violeta Cordon-Preciado, Ji-Young Hwang, Andrés Clemente-Blanco, Pranav Ullal, Jordi Torres-Rosell, Sarah Farmer, …

Nature cell biology, Vol.8(9), pp.1032-1034

09/2006

DOI: https://doi.org/10.1038/ncb1466

PMCID: PMC4493748

PMID: 16892052

Abstract

DNA double-strand breaks (DSB) can arise during DNA replication, or after exposure to DNA-damaging agents, and their correct repair is fundamental for cell survival and genomic stability. Here, we show that the Smc5-Smc6 complex is recruited to DSBs de novo to support their repair by homologous recombination between sister chromatids. In addition, we demonstrate that Smc5-Smc6 is necessary to suppress gross chromosomal rearrangements. Our findings show that the Smc5-Smc6 complex is essential for genome stability as it promotes repair of DSBs by error-free sister-chromatid recombination (SCR), thereby suppressing inappropriate non-sister recombination events.

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Title: Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination
Creators: Andrés Aguilera - Departamento de Genética, Facultad de Biología, Universidad de Sevilla
Gregory Ira - Rosenstiel Center, Brandeis University Baylor College of Medicine, Department of Human and Molecular Genetics
Audrey Ceschia - Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London
Stefan Uhle - Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London
Violeta Cordon-Preciado - Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London
Ji-Young Hwang - Genome Instability Section, Genetics & Molecular Biology Branch, National Human Genome Research Institute, NIH
Andrés Clemente-Blanco - Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London
Pranav Ullal - Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London
Jordi Torres-Rosell - Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London Dept. Ciències Mèdiques Bàsiques, Facultat de Medicina, Universitat de Lleida
Sarah Farmer - Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London
Beatriz Leitao - Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London
Kyungjae Myung - Genome Instability Section, Genetics & Molecular Biology Branch, National Human Genome Research Institute, NIH
Alexandra McAleenan - Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London
Luis Aragón - Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London
Felipe Cortes-Ledesma - Departamento de Genética, Facultad de Biología, Universidad de Sevilla
Debra Bressan - Rosenstiel Center, Brandeis University
Alma Papusha - Baylor College of Medicine, Department of Human and Molecular Genetics
Farokh Dotiwala - Rosenstiel Center, Brandeis University
Felix Machin - Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London
Adam Jarmuz - Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London
Giacomo De Piccoli - Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London
Felip Vilella-Mitjana - Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London
Xiaolan Zhao - Molecular Biology Program, Memorial Sloan-Kettering Cancer Center
James E Haber - Rosenstiel Center, Brandeis University
Publication Details: Nature cell biology, Vol.8(9), pp.1032-1034
Identifiers: 9923958274101921
Academic Unit: Rosenstiel Basic Medical Sciences Research Center; Department of Biology
Language: English
Resource Type: Journal article

Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination

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