Synthesis, characterization and optimization of a two-step immobilized lipase

Kang Zhao; Xi Cao; Qinjian Di; Meng Wang; Hao Cao; Li Deng; Junfeng Liu; Fang Wang; Tianwei Tan

doi:10.1016/j.renene.2016.11.035

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Synthesis, characterization and optimization of a two-step immobilized lipase

Journal article

Peer reviewed

Synthesis, characterization and optimization of a two-step immobilized lipase

Kang Zhao, Xi Cao, Qinjian Di, Meng Wang, Hao Cao, Li Deng, Junfeng Liu, Fang Wang and Tianwei Tan

Renewable energy, Vol.103, pp.383-387

04/2017

DOI: https://doi.org/10.1016/j.renene.2016.11.035

Abstract

Immobilization

Mechanisms

SEM

Lipase

Biocatalyst

Lipase (Candida sp. 99–125) was immobilized by a two-step process, adsorption and subsequent entrapment. The formation mechanism of immobilized lipase was investigated by SEM and EDS. With the support of diatomite, the internal network of immobilized lipase showed more porosity and was fully developed. The dimension of the network was more uniform in the present of gelatine. The maximum biodiesel yield was 92% at 40 °C in a 24 h reaction. The biodiesel yield was maintained at about 70% after 11 repeated batch reactions. This enzyme immobilization method has potential applications in biodiesel and other lipase catalyzed synthesis process.

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Title: Synthesis, characterization and optimization of a two-step immobilized lipase
Creators: Kang Zhao - Beijing Bioprocess Key Laboratory, National Energy R&D Center for Biorefinery, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China
Xi Cao - Beijing Bioprocess Key Laboratory, National Energy R&D Center for Biorefinery, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China
Qinjian Di - Beijing Bioprocess Key Laboratory, National Energy R&D Center for Biorefinery, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China
Meng Wang - Beijing Bioprocess Key Laboratory, National Energy R&D Center for Biorefinery, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China
Hao Cao - Beijing Bioprocess Key Laboratory, National Energy R&D Center for Biorefinery, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China
Li Deng - Beijing Bioprocess Key Laboratory, National Energy R&D Center for Biorefinery, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China
Junfeng Liu - Beijing Bioprocess Key Laboratory, National Energy R&D Center for Biorefinery, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China
Fang Wang - Beijing Bioprocess Key Laboratory, National Energy R&D Center for Biorefinery, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China
Tianwei Tan - Beijing Bioprocess Key Laboratory, National Energy R&D Center for Biorefinery, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China
Publication Details: Renewable energy, Vol.103, pp.383-387
Publisher: Elsevier Ltd
Identifiers: 9923972832001921
Academic Unit: Department of Chemistry
Language: English
Resource Type: Journal article

Synthesis, characterization and optimization of a two-step immobilized lipase

Abstract

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