Synthesis of in-situ formable hydrogels with collagen and hyaluronan through facile Michael addition

Riwang Li; Zhengwei Cai; Zhiwen Li; Qian Zhang; Shuyun Zhang; Li Deng; Lu Lu; Lihua Li; Changren Zhou

doi:10.1016/j.msec.2017.04.046

Back

Journal article

Synthesis of in-situ formable hydrogels with collagen and hyaluronan through facile Michael addition

Riwang Li, Zhengwei Cai, Zhiwen Li, Qian Zhang, Shuyun Zhang, Li Deng, Lu Lu, Lihua Li and Changren Zhou

Materials science & engineering. C, Materials for biological applications, Vol.77, pp.1035-1043

08/01/2017

DOI: https://doi.org/10.1016/j.msec.2017.04.046

PMID: 28531976

Abstract

Hyaluronic Acid

Cell Culture Techniques

Hydrogels - chemistry

Extracellular Matrix

Collagen

To mimic the natural extracellular matrix (ECM) and to facilitate tissue regeneration, in-situ formable collagen/hyaluronan composite hydrogels were prepared by a facile approach via Michael addition reaction, with maleilated collagen (Col-MA) and thiol derivatized hyaluronan (HA-SH). The hydrogels were denoted as CHG-1, CHG-2, CHG-3 and CHG-4 by vinyl/free thiol molar ratio (f) of 1:1, 1:2, 1:3 and 1:4, respectively. Results showed that with decrease of the f values, the gelation time decreased from 43s to 15s, Young's modulus increased from 1671.65Pa to 9105.86Pa, and the swelling ratio increased from 1.5 to 12.7. SEM images of the air-dried samples revealed that the chemical modification process did not denature the collagen, and the interwoven collagen fibrils were indeed observed. Cell culture confirmed that the CHG facilitated the growth and proliferation of MC3T3-E1. Cells displayed spreading morphology and formed nearly aligned cell layers with the extension of culture time. Therefore, it is suggested that CHG can be used as injectable materials for tissue regeneration.

Metrics

32 Record Views

Details

Title: Synthesis of in-situ formable hydrogels with collagen and hyaluronan through facile Michael addition
Creators: Riwang Li - Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China
Zhengwei Cai - Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China
Zhiwen Li - Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China
Qian Zhang - Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China
Shuyun Zhang - Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China
Li Deng - Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China
Lu Lu - Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China
Lihua Li - Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China. Electronic address: tlihuali@jnu.edu.cn
Changren Zhou - Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou 510632, China
Publication Details: Materials science & engineering. C, Materials for biological applications, Vol.77, pp.1035-1043
Publisher: Netherlands
Identifiers: 9923972795201921
Academic Unit: Department of Chemistry
Language: English
Resource Type: Journal article

Synthesis of in-situ formable hydrogels with collagen and hyaluronan through facile Michael addition

Abstract

Metrics

Details

Brandeis University Social media