The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility

Lillian K Fritz-Laylin; Simon E Prochnik; Michael L Ginger; Joel B Dacks; Meredith L Carpenter; Mark C Field; Alan Kuo; Alex Paredez; Jarrod Chapman; Jonathan Pham; Shengqiang Shu; Rochak Neupane; Michael Cipriano; Joel Mancuso; Hank Tu; Asaf Salamov; Erika Lindquist; Harris Shapiro; Susan Lucas; Igor V Grigoriev; W. Zacheus Cande; Chandler Fulton; Daniel S Rokhsar; Scott C Dawson

doi:10.1016/j.cell.2010.01.032

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The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility

Journal article

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The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility

Lillian K Fritz-Laylin, Simon E Prochnik, Michael L Ginger, Joel B Dacks, Meredith L Carpenter, Mark C Field, Alan Kuo, Alex Paredez, Jarrod Chapman, Jonathan Pham, …

Cell (Cambridge), Vol.140(5), pp.631-642

03/05/2010

DOI: https://doi.org/10.1016/j.cell.2010.01.032

PMID: 20211133

Abstract

CELLBIO

EVO_ECOL

Genome sequences of diverse free-living protists are essential for understanding eukaryotic evolution and molecular and cell biology. The free-living amoeboflagellate Naegleria gruberi belongs to a varied and ubiquitous protist clade (Heterolobosea) that diverged from other eukaryotic lineages over a billion years ago. Analysis of the 15,727 protein-coding genes encoded by Naegleria's 41 Mb nuclear genome indicates a capacity for both aerobic respiration and anaerobic metabolism with concomitant hydrogen production, with fundamental implications for the evolution of organelle metabolism. The Naegleria genome facilitates substantially broader phylogenomic comparisons of free-living eukaryotes than previously possible, allowing us to identify thousands of genes likely present in the pan-eukaryotic ancestor, with 40% likely eukaryotic inventions. Moreover, we construct a comprehensive catalog of amoeboid-motility genes. The Naegleria genome, analyzed in the context of other protists, reveals a remarkably complex ancestral eukaryote with a rich repertoire of cytoskeletal, sexual, signaling, and metabolic modules. [Display omitted] [Display omitted] ► Naegleria's genome sequence suggests a versatile and complex ancestral eukaryote ► The genome encodes both aerobic and anaerobic hydrogen-producing metabolism ► We predict novel evolutionarily conserved genes associated with amoeboid motility ► Genomic comparisons identify 4133 protein families likely ancestral to all eukaryotes

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Title: The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility
Creators: Lillian K Fritz-Laylin - Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
Simon E Prochnik - U.S. Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, USA
Michael L Ginger - School of Health and Medicine, Division of Biomedical and Life Sciences, Lancaster University, Lancaster LA1 4YQ, UK
Joel B Dacks - Department of Cell Biology, University of Alberta Edmonton, Alberta, Canada
Meredith L Carpenter - Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
Mark C Field - The Molteno Building, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
Alan Kuo - U.S. Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, USA
Alex Paredez - Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
Jarrod Chapman - U.S. Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, USA
Jonathan Pham - Department of Microbiology, University of California, Davis, CA 95616, USA
Shengqiang Shu - U.S. Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, USA
Rochak Neupane - Center for Integrative Genomics, 545 Life Sciences Addition, University of California, Berkeley, Berkeley, CA 94720, USA
Michael Cipriano - Department of Microbiology, University of California, Davis, CA 95616, USA
Joel Mancuso - Gatan Inc., 5794 W. Las Positas Boulevard, Pleasanton, CA 94588, USA
Hank Tu - U.S. Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, USA
Asaf Salamov - U.S. Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, USA
Erika Lindquist - U.S. Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, USA
Harris Shapiro - U.S. Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, USA
Susan Lucas - U.S. Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, USA
Igor V Grigoriev - U.S. Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, USA
W. Zacheus Cande - Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
Chandler Fulton - Department of Biology, Brandeis University, Waltham, MA 02454-9110, USA
Daniel S Rokhsar - Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
Scott C Dawson - Department of Microbiology, University of California, Davis, CA 95616, USA
Publication Details: Cell (Cambridge), Vol.140(5), pp.631-642
Publisher: Elsevier Inc
Identifiers: 9923980284901921
Academic Unit: Department of Biology
Language: English
Resource Type: Journal article

The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility

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