Abstract
Animals rely on highly sensitive thermoreceptors to seek out optimal temperatures,\r but the molecular mechanisms of thermosensing are not well understood. The Dorsal Organ Cool\r Cells (DOCCs) of the Drosophila larva are a set of exceptionally thermosensitive neurons critical for larval cool avoidance. Here, we show that DOCC cool-sensing is mediated by Ionotropic Receptors (IRs), a family of sensory receptors widely studied in invertebrate chemical sensing. We find that two IRs, IR21a and IR25a, are required to mediate DOCC responses to cooling and are required for cool avoidance behavior. Furthermore, we find that ectopic expression of IR21a can confer cool responsiveness in an Ir25a-dependent manner, suggesting an instructive role for IR21a in\r thermosensing. Together, these data show that IR family receptors can function together to\r mediate thermosensation of exquisite sensitivity.