Abstract
Inducible cat genes from Gram‐positive bacteria are regulated by translation attenuation. The inducer chloramphenicol stalls a ribosome at a specific site in the leader of cat transcripts; this destabillzes a downstream stem‐loop structure that normally sequesters the ribosome‐binding site for the cat structural gene. The five‐amino‐acid peptide MVKTD that is synthesized when a ribosome has translated to the leader induction site is an inhibitor of peptidyl transferase In vitro. Thus, the peptide may be the in vivo determinant of the site of ribosome stalling. Here we provide evidence that the leader pentapeptide can exert a cis‐effect on its translating ribosome In vivo. Converting leader codon 6 to the ochre codon results in expression of cat‐86 in the absence of Inducer. We term this autoinduction. Autoinduction is abolished by mutations that change the amino‐acid sequence of the leader peptide but have no, or little, effect on the sequence of nucleotides at the leader stall site. In contrast, four nucleotide changes within the leader site occupied by the stalled ribosome that result in synonymous codon replacements do not diminish autoinduction. Our evidence indicates that the cat‐86 leader pentapeptide can alter the function of its translating ribosome.