Abstract
LATE-PCR is a method for amplifying 10-20 fold more single-stranded DNA than double-stranded DNA under closed-tube conditions. LATE-PCR has many applications in the fields of diagnostics and DNA barcoding, but has never been used as a tool to generate single-strands that can be used, in turn, to construct nanoscale structures. Our goal, using the basic principles of DNA hybridization, was to couple LATE-PCR amplification to DNA assembly, thereby contributing a new approach to the field of DNA origami. In this thesis, we present a strategy, as well as initial experimental steps and challenges in using LATE-PCR for construction of hexagons from DNA. The results from this study indicate that LATE-PCR has the potential to build complex DNA structures given the right experimental conditions.