Abstract
DNA origami represents a revolutionary nanotechnology that harnesses the programmability of single stranded DNA to construct complex and precisely controlled nanostructures. Through complementary base pairing, single-stranded DNA is folded into predefined shapes using a DNA scaffold strand and many shorter staple strands. This level of nanometer-scale precision enables the creation of custom shapes, patterns, and even intricate three- dimensional structures that have implications across diverse scientific disciplines, including drug delivery and biological research. Octahedral DNA origami, known for its robustness and expanded surface area, holds promise for multifunctional applications. In our study, we explore the self-assembly dynamics of octahedral DNA origami within complex environments similar to blood vessels, utilizing polyethylene glycol (PEG) as a mimetic polymer solution. PEG, valued for its biocompatibility and stability, provides an optimal setting for investigating DNA origami behavior under crowded conditions. Our experiments involve varying PEG polymer sizes and concentrations, mimicking the complex conditions of biomolecules and macromolecules found within biological systems. By probing the dynamics of octahedral DNA origami nucleation and crystal growth, we aim to uncover the intricate processes governing its self-assembly. This enhanced understanding holds the potential to transform nanoscale device design and propel DNA nanotechnology towards innovative frontiers.