Abstract
Infantile Spasms is a seizure disorder appearing in young children and is characterized by myoclonic jerks. Organotypic brain slices from mouse pups were exposed to tetrodotoxin (TTX) for five days, then fixed and imaged using the CLARITY method. TTX-treated slices were found to have a larger area of parvalbumin-positive puncta than control slices. This suggests a role for activity in synaptic pruning.